Publications

Peer-Reviewed Publications from Postdoctoral Research (2020-2026)

1. Goul, C., Yang, S., Bonini, S., Aleksashin, N., Citron, R., Shin, H.R., Cate, J., Winter, D., Zoncu, R. (2026) LSG1-VAP binding promotes 60S Large Ribosomal Subunit Assembly, SciELO Preprints, https://preprints.scielo.org/index.php/scielo/preprint/view/14782

2. Aleksashin N.A., Shelke R.R., Yin T., Cate JHD (2025) Overcoming the eIF2α Brake in Human Cell-Derived Translation Systems, BioRxiv, doi: https://doi.org/10.1101/2025.11.16.688697; https://www.biorxiv.org/content/10.1101/2025.11.16.688697v1

3. Aleksashin N.A., Langeberg J.C., Shelke R.R., Yin T., Cate JHD (2025) RNA elements required for the high efficiency of West Nile Virus-induced ribosomal frameshifting, Nucleic Acids Research, 24;53(3):gkae1248.

4. Aleksashin N.A., Chang ST-L, Cate J. (2023) A highly efficient human cell-free translation system. RNA https://doi.org/10.1261/rna.079825.123.

Peer-Reviewed Publications from Graduate Research (2014-2019)

5. Zhang, Y. , Aleksashin, N.A., Klepacki, D., Anderson, C., Vazquez-Laslop, N., Gross, C.A., Mankin, A.S. (2022) The context of the ribosome binding site in mRNAs defines the specificity of action of kasugamycin, an inhibitor of translation initiation. Proc Natl Acad Sci USA. 119(4):e2118553119. doi:10.1073/pnas.2118553119.

6. Huang, S., Aleksashin, N.A., Loveland, A.B., Klepacki, D., Reier, K., Kefi, A., Szal, T., Remme, J., Jaeger, L., Vazquez-Laslop, N., Korostelev, A.A., Mankin, A.S. (2020) Ribosome engineering reveals the importance of 5S rRNA autonomy for ribosome assembly. Nat Commun 11(1):2900. doi: 10.1038/s41467-020-16694-8.

7. Aleksashin, N.A., Szal, T., d’Aquino, A.E., Jewett, M.C., Mankin, A.S. (2020) A fully orthogonal system for protein synthesis in bacterial cells. Nat Commun 11, 1858. https://doi.org/10.1038/s41467-020-15756-1

8. d’Aquino, A.E., Azim, T., Aleksashin, N.A., Hockenberry, A.J., Kruger, A., Jewett, M.C. (2020) Mutational characterization and mapping of the 70S ribosome active site. Nucleic Acids Res. 18;48(5):2777-2789. doi: 10.1093/nar/gkaa001.

9. Aleksashin, N.A., Leppik, M., Hockenberry, A.J., Klepacki, D., Vazquez-Laslop, N., Jewett, M.C., Remme, J., Mankin, A.S. (2019) Assembly and functionality of the ribosome with tethered subunits. Nat Commun. 10(1):930. doi: 10.1038/s41467-019-08892-w.

10. Polikanov, Y.S., Aleksashin, N.A., Beckert, B., Wilson, D.N. (2018) The Mechanisms of Action of Ribosome-Targeting Peptide Antibiotics. Front Mol Biosci. 5:48. doi: 10.3389/fmolb.2018.00048. eCollection 2018.

11. Sergiev, P.V., Aleksashin, N.A., Chugunova, A.A., Polikanov, Y.S., Dontsova, O.A. (2018) Structural and evolutionary insights into ribosomal RNA methylation. Nat Chem Biol. 14(3):226-235. doi:10.1038/nchembio.2569.